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Immunocytochemistry Immunolabeling of NPCs and derived neurons was accomplished by utilization of a standard two-day protocol with: 15 min fixation in 4% PFA, 10 min permeabilization in 0.1% triton-PBS and 30 min blocking with 5% donkey serum

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TB-500 reduces systemic inflammation by downregulating pro-inflammatory cytokines, including NF-kB, allowing tissues to transition from the inflammatory phase of healing into the proliferative phase more rapidly